Anti-CD14/FITC抗体使用说明书

Anti-CD14/FITC抗体使用说明书

Catalog Number : BYK-1192R-FITC      
Quantity size :   100ug (2mg/1ml, Lyophilized or Liquid. Buffer = 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide. Reconstitute with 50ul sterile distilled water.)

Background:  CD14 is a single copy gene encoding 2 protein forms: a 50 to 55 kDa glycosylphosphatidylinositol anchored membrane protein (mCD14) and a monocyte or liver derived soluble serum protein (sCD14) that lacks the anchor. Both molecules are critical for lipopolysaccharide (LPS) dependent signal transduction, and sCD14 confers LPS sensitivity to cells lacking mCD14. Increased sCD14 levels are associated with inflammatory infectious diseases and high mortality in gram negative shock. CD14 also appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Also known as: CD14 antigen; CD14 molecule; Lipopolysaccharide receptor; LPSR; Monocyte Differentiation Antigen 14; Monocyte differentiation antigen CD14; Myeloid cell specific leucine rich glycoprotein; CD14_MOUSE; Myeloid cell-specific leucine-rich glycoprotein.

Specificity:   -Rabbit Polyclonal IgG, affinity purified by Protein A.

-FITC conjugated

-Reacts with: Human, Mouse, Rat, Dog, Pig, Cow, Rabbit, Sheep,  

-Immunogen: KLH conjugated synthetic peptide derived from human Ki67.

-Predicted molecular weight: 40kDa

Application:  ICC=1:50-200 IF=1:50-200

            Excitation spectrum: 490-495nm

            Emission spectrum: 520-530nm

            Not yet tested in other applications.

            Optimal working dilutions must be determined by the end user.

Storage:     Store at -20 °C for one year. Avoid repeated freeze/thaw

            cycles. The Lyophilized or Liquid antibody is stable at room

            temperature for at least one month and for greater than a year when

            kept at -20 °C. When reconstituted in sterile pH 7.4 0.01M PBS or

            diluent of antibody the antibody is stable for at least two weeks at

            2-4 °C.

Important Note: This product as supplied is intended for research

             use only, not for use in human, therapeutic or diagnostic

             applications.