鸡5核苷酸酶（5-NT）ELISA试剂盒不买后悔 返回列表页

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产品名称：鸡5核苷酸酶（5-NT）ELISA试剂盒不买后悔    
英文名称：Chicken 5-Nucleotidase,5-NT  ElisaKit.
产品编号：BYS12687B
产品性状：液体
产品用途：科研实验
产品规格：96T/48T

ELISA实验原理: 用纯化的抗体包被微孔板，制成固相载体，往包被抗体的微孔中依次加入标本或者标准品、*化的抗体、HRP-标记的亲和素，经过*洗涤后用底物显色。底物在过氧化物酶的催化下转化成蓝色， 并在酸的作用下转化成zui终的黄色。 颜色的深浅和样品中的目标蛋白呈正相关。用酶标仪在（450nm）波长下测定测定O.D.值（吸光度），计算样品浓度。.

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The microtiter plate provided in ELISA Kit has been precoated with an antibody specific to the target protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated specific polyclonal antibody preparation,and Avidincon jugated to Horseradish Peroxidase（HRP） is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve