服务承诺:
工作时间内提供免费的技术咨询和指导 。我公司的销售工程师经过专业培训的,将为您提供满意的产品和真诚的服务。
葡萄糖酸铜 号:527-09-3
英文名称:Copper gluconate;Copper(II) gluconate;D-Gluconic acid copper(II) salt
其他名称:双(D-葡糖酸)铜;葡萄糖酸铜(II)
号:527-09-3或13005-35-1
C12H22CuO14=453.84
级别:BR
纯度:≥98.0%
干燥失重:≤2.0%
性状(以下信息仅供参考):白色或浅蓝色至蓝绿色结晶或粉末,无臭,极易溶于水,难溶于乙醇
用途:本品仅供科研,不得用于其它用途。
保存:RT 葡萄糖酸铜 号:527-09-3储存条件:
避光、干燥阴凉处封闭贮存,严禁与有毒、有害物品混放、混运。本品为非危险 产品可按一般化学品运输,轻搬动轻放,防止日晒、雨淋!受热、受潮、受光后易丧失活力,保存期短,因此贮存和运输条件比较苛刻。
运输:汽车运输、EMS邮政快递,申通快递等, 款到上海3天内发货;
售后:如您对我们的产品服务及技术指标有特殊要求,请及时通知我方。
存储:应贮存在干燥清洁避光的环境中,严禁与有毒物质混放,以免污染(保质期为两年)。
葡萄糖酸铜 号:527-09-3主要优级纯、分级纯和化学纯3种:
(1)优级纯(GR:Guaranteed reagent),又称一级品或保证试剂,99.8%,这种试剂纯度Z高,杂质含量Z低,适合于重要精密的分析工作和科学研究工作,使用绿色瓶签。
(2)分析纯(AR),又称二级试剂,纯度很高,99.7%,略次于优级纯,适合于重要分析及一般研究工作,使用红色瓶签。
(3)化学纯(CP),又称三级试剂,≥ 99.5%,纯度与分析纯相差较大,适用于工矿、学校一般分析工作。使用蓝色(深蓝色)标签。
(4)实验试剂(LR:Laboratory reagent),又称四级试剂。
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.
Function : SirtT1 75 kDa fragment: catalytically inactive 75SirT1 may be involved in regulation of apoptosis. May be involved in protecting chondrocytes from apoptotic death by associating with cytochrome C and interfering with apoptosome assembly.
Subunit : Found in a complex with PCAF and MYOD1 (By similarity). Component of the eNoSC complex, composed of SIRT1, SUV39H1 and RRP8. Interacts with HES1, HEY2 and PML. Interacts with RPS19BP1/AROS. Interacts with KIAA1967/DBC1 (via N-terminus); the interaction disrupts the interaction between SIRT1 and p53/TP53. Interacts with SETD7; the interaction induces the dissociation of SIRT1 from p53/TP53 and increases p53/TP53 activity. Interacts with MYCN, NR1I2, CREBZF, TSC2, TLE1, FOS, JUN, NR0B2, PPARG, NCOR, IRS1, IRS2 and NMNAT1. Interacts with HNF1A; the interaction occurs under nutrient restriction. Interacts with SUZ12; the interaction mediates the association with the PRC4 histone methylation complex which is specific as an association with PCR2 and PCR3 complex variants is not found. Interacts with -1 tat.
Subcellular Location : Nucleus, PML body. Cytoplasm. Note=Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. Shuttles between nucleus and cytoplasm.
SirtT1 75 kDa fragment: Cytoplasm. Mitochondrion.
Tissue Specificity : Widely expressed.
Post-translational modifications : Methylated on multiple lysine residues; methylation is enhanced after DNA damage and is dispensable for deacetylase activity toward p53/TP53.
Phosphorylated. Phosphorylated by STK4/MST1, resulting in inhibition of SIRT1-mediated p53/TP53 deacetylation. Phosphorylation by MAPK8/JNK1 at Ser-27, Ser-47, and Thr-530 leads to increased nuclear localization and enzymatic activity. Phosphorylation at Thr-530 by DYRK1A and DYRK3 acivates 葡萄糖酸铜 号:527-09-3deacetylase activity and promotes cell survival. Phosphorylation by mammalian target of rapamycin complex 1 (mTORC1) at Ser-47 inhibits deacetylation activity. Phosphorylated by CaMK2, leading to increased p53/TP53 and NF-kappa-B p65/RELA deacetylation activity (By similarity). Phosphorylation at Ser-27 implicating MAPK9 is linked to protein stability. There is some ambiguity for some phosphosites: Ser-159/Ser-162 and Thr-544/Ser-545.
Proteolytically cleaved by cathepsin B upon TNF-alpha treatment to yield catalytic inactive but stable SirtT1 75 kDa fragment (75SirT1).
S-nitrosylated by GAPDH, leading to inhibit the NAD-dependent protein deacetylase activity (By similarity).
Similarity : Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain.
Database links : UniProtKB/Swiss-Prot: Q96EB6.2
在Sirtuin蛋白家族中,sirtuin 1参与多种新陈代谢活动,包括DNA的自我保护和修复,抑制脂质过氧化积累,抑制其他细胞凋亡相关基因的表达以及和细胞寿命相关的活动。限制摄入的热量可以加强SIRT1的表达,从而延长了寿命。
英文名称 Anti-Smac/DIABLO
中文名称 线粒体促凋亡蛋白抗体
别 名 0610041G12Rik; DBLOH_HUMAN; DBOH; DBOH; diablo; Diablo homolog (Drosophila); Diablo homolog (Drosophila); Diablo homolog; Diablo homolog mitochondrial; DIABLO S; DIABLO S; Direct IAP binding protein with low pI; Direct IAP binding protein with low pI; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; mitochondrial; Mitochondrial Smac protein; Second mitochondria derived activator of caspase; Second mitochondria derived activator of caspase; Second mitochondria-derived activator of caspase; SMAC 3; Smac; Smac protein; Smac protein; SMAC3; SMAC3;
浓 度 1mg/1ml
规 格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse
产品类型 一抗
研究领域 肿瘤 细胞生物 细胞凋亡 新陈代谢 线粒体
蛋白分子量 predicted molecular weight: 21kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated synthetic peptide derived from human Smac C-terminus
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 This gene encodes an inhibitor of apoptosis protein (IAP)-binding protein. The encoded mitochondrial protein enters the cytosol when cells undergo apoptosis, and it moderates the caspase inhibition of IAPs. Multiple polyadenylation sites have been found for this gene. Several alternatively spliced transcript variants that encode distinct isoforms have been described for this gene but the validity of some transcripts, and their predicted ORFs, has not been determined conclusively. The inhibitor of apoptosis (IAP) proteins regulate programmed cell death by inhibiting members of the caspase family of enzymes. A novel mammalian protein that binds to IAPs and neutralizes their inhibitory effect on caspases has been designated Smac/DIABLO. This is a mitochondrial protein that is released along with cytochrome c during apoptosis and activates the cytochrome c/Apaf-1/caspase-9 pathway. Analysis of the structural basis of Smac/DIABLO reveals that the N-terminal amino acids are required for binding of Smac/DIABLO to IAPs and activation of caspases. Smac/DIABLO is expressed in a variety of human and mouse tissues.
Function : Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis-inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
Subunit : Homodimer. Interacts with NGFRAP1/BEX3 (By similarity). Interacts with BIRC2/c-IAP1, BIRC3/c-IAP2, XIAP/BIRC4, BIRC6/bruce and BIRC7/livin. Interacts with the monomeric and dimeric form of BIRC5/survivin.
Subcellular Location : Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis.
Tissue Specificity : Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed.
Post-translational modifications : Ubiquitinated by BIRC7/livin.
DISEASE : Deafness, autosomal dominant, 64 (DFNA64) [MIM:614152]: A form of non-syndromic sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Note=The disease is caused by mutations affecting the gene represented in this entry.
Database links :
UniProtKB/Swiss-Prot: Q9NR28.1
Entrez Gene: 56616 Human
Entrez Gene: 66593 Mouse
Entrez Gene: 288753 Rat
Omim: 605219 Human
SwissProt: Q9NR28 Human
SwissProt: Q9JIQ3 Mouse
Unigene: 169611 Human
Unigene: 46716 Mouse
Smac(second mitochondria-derived activator of caspase)又称DIABLO(direct I