产品名称:10×多聚赖氨酸L型溶液售后服务
英文名字:10xPoly-L-lysine solution
产品描述
多聚赖氨酸(L型)英文名为Poly-L-lysine,简称PLL。本Poly-L-lysine为Poly-L-lysine hydrobromide,分子式为L-Lys-(L-Lys)n-L-Lys•xHBr,分子量为150,000-300,000, Number 25988-63-0。
多聚赖氨酸溶液是广泛应用的组织切片与玻片黏合剂,该多聚阳离子分子与组织切片上的阴离子相互作用会产生较强的黏合力。
本产品为已经配制好的多聚赖氨酸L型溶液,并经过滤除菌处理,可以直接稀释后用于细胞或组织培养方面的实验。多聚赖氨酸溶液,一般的使用浓度为0.01%。本品为10×多聚赖氨酸L型溶液,使用时用灭菌的双蒸水稀释至1×后使用。
应用
本品适用于组织学,免疫组织化学,冻切片,细胞涂片,原位杂交等实验中玻片的防脱片处理,以防实验操作过程中组织掉片。也可用于细胞培养,增加细胞贴壁能力。
储存条件
-20℃保存,避免反复冻融。
10×多聚赖氨酸L型溶液售后服务注意事项
1. 每 100ml 已稀释的多聚赖氨酸溶液可处理 90 张玻片,超过 90 张玻片会影响其黏合力。
2. 包被之前玻片必须保持清洁,必要时用含 1%HCl 的 70%乙醇溶液来清洗。
3. 稀释过的多聚赖氨酸溶液可放在2-8℃,至少3个月内是稳定的。
4. 用过的稀释液再次使用时要过滤,若出现浑浊或染菌应丢弃。
5. Poly-L-lysine和Poly-D-lysine都可以用于促进细胞的贴壁生长。Poly-L-lysine可以被某些细胞所消化并吸收,摄入过多的Poly-L-lysine会产生一定的细胞毒性。如果遇到Poly-L-lysine有细胞毒性的情况,可以考虑选购Poly-D-lysine。
6. 为了您的安全和健康,请穿实验服并戴一次性手套操作。
10×多聚赖氨酸L型溶液售后服务产品应用:
对于一些相对特殊的产品,我们会提供产品说明书,里面会对产品溶解性,及我们所能了解的产品应用信息,实验方案进行描述,您可以作为参考。对于常见试剂,因为应用广泛,一般不提供说明书。如果您在实验过程中遇到了问题,欢迎与我们讨论,我们将尽力帮您解决。
10×多聚赖氨酸L型溶液售后服务分装及溶液配制:
您收到货物后不要自己进行分包,因为分包环境、包装材料等因素可能导致分包后的产品变质;如您有特殊包装要求,请在订购时候与我们客服代表阐明,当然价格会做适当调整。对于开盖后,长期未使用的,请务必重新密封好,建议Parafilm封口膜,并按照相应储存条件使用。如果放置时间过长,超过产品有效期,建议您重新购买,以免影响实验质量。
10×多聚赖氨酸L型溶液售后服务储存条件:
大部分的试剂产品是比较稳定的,只要您严格按照产品包装或者说明书上的储存条件进行储存,*可以保证其在有效期内的正常使用。一般常见储存条件有零下XX摄氏度,2-8摄氏度,常温等。特别提醒,对于某些酶类制品,如果储存条件是2-8摄氏度,不要储存在零下,否则容易失活,对于某些需要严格避光,防潮,充氮气保护的,接触空气或者过分暴露非常容易氧化或者受潮变质。
低温储存产品,运输途中冷藏问题:
对于需要低温储存的产品,我们在运输时候都会采用冷藏包装,但是部分由于外界气温过高,运输时间较长,对于普通冷藏包装,途中可能会温度升高,这些都是没有问题的,因为这种普通冷藏只适用于那些短期内(2周)高温依然稳定的产品,建议低温的储存条件,只是长期储存时候的条件,您可以放心使用。对于某些特殊的产品,如抗体,我们会采用干冰冷藏包装,确保全程低温。公司都对产品性状进行了严格的加速稳定测试,行业经验丰富。
10×多聚赖氨酸L型溶液售后服务储存条件:避光、干燥阴凉处封闭贮存,严禁与有毒、有害物品混放、混运。本品为非危险 产品可按一般化学品运输,轻搬动轻放,防止日晒、雨淋!受热、受潮、受光后易丧失活力,保存期短,因此贮存和运输条件比较苛刻。
CFN99589 http://www.chemfaces。。com/natural/Oxypaeoniflorin-CFN99589.html C23H28O12 496.46 39011-91-1 Oxypaeoniflorin Monoterpenoids >=98% 10mg
CFN99591 http://www.chemfaces。。com/natural/Pseudohypericin-CFN99591.html C30H16O9 520.44 55954-61-5 Pseudohypericin Anthraquinones >=98% 20mg
CFN99592 http://www.chemfaces。。com/natural/Rhein-8-O-D-glucopyranoside-CFN99592.html C21H16O11·Ca 484.43 113443-70-2 Rhein-8-glucoside calcium salt Anthraquinones >=98% 20mg
CFN99593 http://www.chemfaces。。com/natural/Sanggenone-C-CFN99593.html C40H36O12 708.71 80651-76-9 Sanggenone C Flavonoids >=98% 20mg
CFN99595 http://www.chemfaces。。com/natural/Senkyunolide-H-CFN99595.html C12H16O4 224.3 94596-27-7 Senkyunolide H Miscellaneous >=98% 20mg
CFN99597 http://www.chemfaces。。com/natural/Sennidin-A-CFN99597.html C30H18O10 538.46 641-12-3 Sennidin A Anthraquinones >=98% 20mg
CFN99598 http://www.chemfaces。。com/natural/Sennidin-B-CFN99598.html C30H18O10 538.46 517-44-2 Sennidin B Anthraquinones >=98% 20mg
CFN99599 http://www.chemfaces。。com/natural/Sinensetin-CFN99599.html C20H20O7 372.37 2306-27-6 Sinensetin Flavonoids >=98% 20mg
CFN99703 http://www.chemfaces。。com/natural/Cyperone-CFN99703.html C15H22O 218.33 473-08-5 alpha-Cyperone Sesquiterpenoids >=98% 20mg
required for acetylation by CREBBP. Phosphorylated on Ser-392 following UV but not gamma irradiation. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylated on Ser-15 upon ultraviolet irradiation; which is enhanced by interaction with BANP. Phosphorylated by NUAK1 at Ser-15 and Ser-392; was initially thought to be mediated by STK11/LKB1 but it was later shown that it is indirect and that STK11/LKB1-dependent phosphorylation is probably mediated by downstream NUAK1 (PubMed:21317932). It is unclear whether AMP directly mediates phosphorylation at Ser-15. Phosphorylated on Thr-18 by isoform 1 and isoform 2 of VRK2. Phosphorylation on Thr-18 by isoform 2 of VRK2 results in a reduction in ubiquitination by MDM2 and an increase in acetylation by EP300. Stabilized by CDK5-mediated phosphorylation in response to genotoxic and oxidative stresses at Ser-15, Ser-33 and Ser-46, leading to accumulation of p53/TP53, particularly in the nucleus, thus inducing the transactivation of p53/TP53 target genes. Phosphorylated at Ser-315 and Ser-392 by CDK2 in response to DNA-damage.
Dephosphorylated by PP2A-PPP2R5C holoenzyme at Thr-55. SV40 small T antigen inhibits the dephosphorylation by the AC form of PP2A.
May be O-glycosylated in the C-terminal basic region. Studied in EB-1 cell line.
Ubiquitinated by MDM2 and SYVN1, which leads to proteasomal degradation. Ubiquitinated by RFWD3, which works in cooperation with MDM2 and may catalyze the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome. Ubiquitinated by MKRN1 at Lys-291 and Lys-292, which leads to proteasomal degradation. Deubiquitinated by USP10, leading to its stabilization. 10×多聚赖氨酸L型溶液售后服务Ubiquitinated by TRIM24, which leads to proteasomal degradation. Ubiquitination by TOPORS induces degradation. Deubiquitination by USP7, leading to stabilization. Isoform 4 is monoubiquitinated in an MDM2-independent manner.
Monomethylated at Lys-372 by SETD7, leading to