背景介绍:The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene
别名:26S protease regulatory subunit 6B; 26S proteasome AAA ATPase subunit RPT3; 26S proteasome AAA-ATPase subunit RPT3; MB67 interacting protein; MB67-interacting protein; MIP224; Protease 26S subunit 6; Proteasome (prosome macropain) 26S subunit ATPase 4; Proteasome 19S S6; Proteasome 26S subunit ATPase 4; Proteasome 26S subunit, ATPase, 4; PRS6B_HUMAN; PSMC4; RPT3; S6; Tat binding protein 7; TAT-binding protein 7; TBP 7; TBP-7.
Tat结合蛋白7抗体,Anti-TBP7抗体简单介绍:
产品编号:BYK-18836R
英文名称:Anti-TBP7
中文名称:Tat结合蛋白7抗体
产品规格:50ul;100ul;200ul;1ml,*
抗体来源 Rabbit or Mouse
克隆类型 Polyclonal or Monoclonal
交叉反应、产品应用 (具体交叉反应与产品应用请或添加客服直接索取)
性 状 Lyophilized or Liquid
浓 度 1mg/ml
Tat结合蛋白7抗体,Anti-TBP7抗体产品信息:
博研生物小课堂:
常用激光器对应荧光素的选择:
蓝色激光器荧光素(488nm):Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7
红色激光器荧光素(633nm):APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647
黄色激光器荧光素(561nm):PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594
流式抗体选择标准
确定流式细胞仪的参数配置
在设计流式方案前,先确定需要使用流式细胞仪检测的型号,对该仪器的参数配置要了解以下几个方面信息:
激光器:常见的流式细胞仪有488nm和635nm/633nm两个激光器,但有些机器在购买时因某些原因,只装了一个488nm激光器,所以需要注意型号相同的机器也未必激光配置相同。
滤光片:也就是有几个检测通道,这决定了你zui多可以同时检测几个指标。
仪器的具体型号:有助于再次确定检测通道。因为同一个荧光在不同的流式细胞仪上检测,有时候检测通道也是不一样的。
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