背景介绍:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximay 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.
别名:Core histone macro h2a.1; Core histone macro-H2A.1; H2A histone family member Y; H2A.y; H2A/y; H2AF12M; H2AFJ; H2afy; H2AY_HUMAN; Histone H2A.Y; Histone macroH2A1; Histone macroH2A1.1; Histone macroH2A1.2; Macroh2a1; MACROH2A1.1; MacroH2A1.2; Medulloblastoma antigen MU MB 50.205; Medulloblastoma antigen MU-MB-50.205; mH2a; mH2A1
异型组蛋白H2A.1抗体,Anti-macro H2A.1抗体简单介绍:
产品编号:BYK-18546R
英文名称:Anti-macro H2A.1
中文名称:异型组蛋白H2A.1抗体
产品规格:50ul;100ul;200ul;1ml,*
抗体来源 Rabbit or Mouse
克隆类型 Polyclonal or Monoclonal
交叉反应、产品应用 (具体交叉反应与产品应用请或添加客服直接索取)
性 状 Lyophilized or Liquid
浓 度 1mg/ml
异型组蛋白H2A.1抗体,Anti-macro H2A.1抗体产品信息:
博研生物小课堂:
常用激光器对应荧光素的选择:
蓝色激光器荧光素(488nm):Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7
红色激光器荧光素(633nm):APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647
黄色激光器荧光素(561nm):PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594
荧光的选择
选择直标还是间标:待测抗原表达高的尽量选择直标抗体,待测抗原表达丰度低的要考虑选间标*标记标抗体。
荧光强弱:荧光本身有强弱之分,可视抗原表达强弱及分群情况选择合适的荧光。如抗原表达弱或分群不明显的建议选择强荧光例如:PE、APC;反之,抗原表达强或分群明显的建议选择zui常用的弱荧光例如FITC即可(常用荧光强弱排序程度为:PE>APC>PE-cy5/Cy5>PerCP/PerCP-Cy5.5/Cy5.5>FITC。
4. 多色荧光搭配的原则
多色分析方案中荧光抗体的选择和搭配,会受到很多因素的制约:
检测通道不能冲突:每个检测通道只能选择1种荧光素,各通道之间的荧光素可以随意搭配(如FL1选择了FITC标记的抗体,就不能再选择Alexa Fluor488标记的抗体;而同组实验的其余抗体选择PE或者APC标记,都是可以随意组合的);
荧光强弱:所选各种荧光素光谱的重叠应当尽量减少,否则将会导致较大的补偿。zui常用的4色荧光搭配是:FITC、PE、PerCP/PerCP-Cy5.5、APC;
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