背景介绍:This gene encodes a protein which shares sequence similarity with other members of the type II chaperonin family. The encoded protein is a centrosome-shuttling protein and plays an important role in cytokinesis. This protein also interacts with other type II chaperonin members to form a complex known as the BBSome, which involves ciliary membrane biogenesis. This protein is encoded by a downstream open reading frame (dORF). Several upstream open reading frames (uORFs) have been identified, which repress the translation of the dORF, and two of which can encode small mitochondrial membrane proteins. Mutations in this gene have been observed in patients with Bardet-Biedl syndrome type 6, also known as McKusick-Kaufman syndrome. Alternative splicing results in multiple transcript variants.
别名:FLJ31823; Megakaryoblastic leukemia 2; megakaryoblastic leukemia 2 protein; MKL/myocardin-like 2; MKL/myocardin-like protein 2; Mkl2; MKL2_HUMAN; MRTF-B; Myocardin-related transcription factor B; NPD001. MKS.
巨核细胞白血病2抗体,Anti-MKL2抗体简单介绍:
产品编号:BYK-18948R
英文名称:Anti-MKL2
中文名称:巨核细胞白血病2抗体
产品规格:50ul;100ul;200ul;1ml,*
抗体来源 Rabbit or Mouse
克隆类型 Polyclonal or Monoclonal
交叉反应、产品应用 (具体交叉反应与产品应用请或添加客服直接索取)
性 状 Lyophilized or Liquid
浓 度 1mg/ml
巨核细胞白血病2抗体,Anti-MKL2抗体产品信息:
博研生物小课堂:
常用激光器对应荧光素的选择:
蓝色激光器荧光素(488nm):Alexa Fluor 488、FITC、PerCP、PerCP-Cy5.5、PE、PE-Cy5.5、PE-Cy5、PE-Cy7
红色激光器荧光素(633nm):APC、Cy5、APC-Cy5.5、APC-Cy7、Alexa Fluor 647
黄色激光器荧光素(561nm):PE、PE-Cy5.5、PE-Cy5、PE-Cy7、Cy3、Alexa Fluor 555、Alexa Fluor 594
常见问题与解答
1. 高荧光强度背景
(1)荧光标记的抗体浓度应该合适,如果浓度过高,背景会因为非特异性相互作用的增加而增加。
(2)在使用*抗体之前,将样品与过量的蛋白一起培育,如小牛血清蛋白(BSA),脱脂干奶酪,或来自于同一寄主的正常血清作为标记的第二抗体。这个步骤通过阻断*抗体和细胞表面或胞内结构的非特异性的交互作用来降低背景。
(3)在使用*抗体之后,将样品与5%至10%的来自于同一寄主的正常血清和作为标记的第二抗体一起培育。这个步骤会减少不必要的第二抗体与*抗体、细胞表面或胞内结构之间的交互作用。
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