PRP-C18 瑞士 Hamilton 原装PRP-C18 液相色谱柱
The Hamilton PRP-C18 is a new HPLC column designed to provide high-efficiency, reversed-phase separations over an extended column life in nearly any phase or pH. The rigid stationary phase has excellent mechanical and thermal stability(> 100°C), does not shrink or swell, and is compley inert to most conditions commonly encountered in reversed-phase chromatography.
The PRP-C18 is a durable and effective column for general purpose HPLC and especially well-suited for specialized applications in the clinical, pharmaceutical, environmental, food, forensics, and life sciences industries.
PRP-C18 瑞士 Hamilton 原装PRP-C18 液相色谱柱
色谱柱参数：
| 固定相 | PRP-C18 |
| 模式 | Reversed-Phase |
| USP 代码 | USP L21 |
| 填料类型 | PS-DVB functionalized with C18 |
| 填料粒度 | 5 μm |
| 孔径 | 100 ? |
| 内径 | 4.6 mm |
| 柱管材质 | 不锈钢 |
| zui大耐压 | 5,000 psi |
| Phase Limits | pH 1-13. 0-99%水, 有机改性剂 |
| 缓冲液强度 | 0.0-0.5N |
| 温度范围 | 5-85°C |
| 柱子恢复 | Run a gradient of 99% water to 99% acetonitrile. Repeat three times |
| Compound Classification | Organics |
PRP-C18色谱柱-----在任意pH值均可实现高效分离
孔径: 100A
材料:C18功能化的PS-DVB
在建立方法时,改变流动相的pH值是非常有效的，尤在分离一些中性胺类化合物或者在碱性条件下分离其他的有机碱类化合物。
尽管近年来有宣称硅胶基C18色谱柱在碱性pH条件下能够稳定工作,但是所有的硅胶基C18色谱柱在pH>6时，测量性能均有所下降,相比在理想条件下使用,色谱柱的使用寿命也显著缩短。
另一方面，聚合物基质色谱柱具有真正的化学稳定性,固定相能够长期耐受浓度高达1M的NaOH和H2SO4 ,且测量性能不会下降。因为在任何pH值下不会出现填料脱落，渗出或溶解现象,所以色谱柱无需考虑流动相状况在整个使用寿命内均可可靠地重复检测。
高pH值的应用
目前超过70%的药物成分为阳离子溶质,在pH<7时,携带正电荷,过去一直很难将其与其他有机碱分离。主要是因为离子化在反相色谱法中起决定性作用,电离胺样品的洗脱窗口十分狭窄;其次带正电荷的溶质和色谱柱固定相上残余的硅醇基发生第二次相互作用,常常导致出现峰值形状不佳,保留时间出现漂移和柱效降低等异常现象,更进一步缩短色谱柱使用寿命。而PRP-C18可以解决这些问题。
快速洗脱
在现代药物研发科学中,常规分析色谱不应该成为瓶颈。趋势是使用包含较小颗粒的较短色谱柱并在较高流速下使用,从而提高生产率。PRP-C18目前*要求 。
订货信息：
| 订货号 | 产品描述 |
| 79477 | Analytical Guard Cartridge Holder, PEEK |
| 32908 | Analytical Guard Cartridge Holder, Stainless Steel |
| 79291 | Analytical Guard Column, PRP-C18 |
| 79641 | Analytical HPLC Column, CUSTOM ORDER (1.0, 2.1, 4.1, 4.6 mm ID) |
| 79643 | Preparative HPLC Column #1, CUSTOM ORDER (21.2, 30 mm ID) |
| 79644 | Preparative HPLC Column #2, CUSTOM ORDER (50 mm ID) |
| 79645 | Preparative HPLC Column #3, CUSTOM ORDER (100 mm ID) |
| 79675 | PRP-C18 5 μm 4.6 x 50 mm |
| 79678 | PRP-C18 12-20 μm 21.2 x 250 mm |
| 79673 | PRP-C18 5 μm 2.1 x 150 mm |
| 79680 | PRP-C18 5 μm 2.1 x 150 mm PEEK |
| 79674 | PRP-C18 5 μm 2.1 x 250 mm |
| 79681 | PRP-C18 5 μm 2.1 x 250 mm PEEK |
| 79672 | PRP-C18 5 μm 2.1 x 50 mm |
| 79679 | PRP-C18 5 μm 2.1 x 50 mm PEEK |
| 79676 | PRP-C18 5 μm 4.6 x 150 mm |
| 79683 | PRP-C18 5 μm 4.6 x 150 mm PEEK |
| 79677 | PRP-C18 5 μm 4.6 x 250 mm |
| 79684 | PRP-C18 5 μm 4.6 x 250 mm PEEK |
| 79682 | PRP-C18 5 μm 4.6 x 50 mm PEEK |
| 79688 | PRP-C18 Analytical Guard Column Replacement Cartridges (5/pk), PEEK |
| 79686 | PRP-C18 Analytical Guard Column Replacement Cartridges (5/pk), Stainless Steel |
| 79687 | PRP-C18 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), PEEK |
| 79685 | PRP-C18 Analytical Guard Column Starter Kit (1 holder, 2 cartridges), Stainless Steel |
| 79690 | PRP-C18 Semiprep/Preparative Guard Column Replacement Cartridges (2/pk), Stainless Steel |
| 79689 | PRP-C18 Semiprep/Preparative Guard Column Starter Kit (1 holder, 1 cartridge), Stainless Steel |
| 79792 | PRP-C18, 10 μm Bulk Resin |
| 79793 | PRP-C18, 12-20 μm Bulk Resin |
| 79791 | PRP-C18, 5 μm Bulk Resin |
| 5095-01 | Semiprep/Preparative Guard Cartridge Holder, Stainless Steel |
| 79918 | Semiprep/Preparative Guard Column, PRP-C18 |
| 79642 | Semipreparative HPLC Column, CUSTOM ORDER (7.0, 7.8, 10 mm ID) |
