● 产品概况/ product introduction
- 货号EK0557
- 产品名称Human ACE ELISA Kit
- 产品描述For quantitative detection of human ACE in cell culture supernates, serum, plasma(heparin) and saliva.
- 免疫原NSO, L30-L1261
- 交叉反应There is no detectable cross-reactivity with other relevant proteins.
- 敏感性＜5pg/ml
- 检测范围0.78ng/ml-50ng/ml
- 基因名称ACE
- 基因全名angiotensin I converting enzyme
- Uniprot IDP12821
- 指标别名ACE 1 | ACE T | Angiotensin converting enzyme | Angiotensin Converting Enzyme 1 | Angiotensinconverting enzyme | Angiotensin-converting enzyme | CD143 antigen | DCP 1 | DCP, DCP1 | Dipeptidyl carboxypeptidase 1 | Dipeptidyl carboxypeptidase I | Kininase II | MVCD3 | P12821 | Peptidyl dipeptidase A
- 背景资料Angiotensin-converting enzyme(ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.
- 显色时间25-30分钟
● 试剂盒内容/ product introduction
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0557-CAP | 96-well plate precoated with anti- human ACE antibody | 1 |
| EK0557-ST | Lyophilized recombinant human ACE standard | 50ng/tubex2 |
| EK0557-DA | Biotinylated anti- human ACE antibody | 130ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| *小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。 | ||
● 需要用户自己提供的器材和试剂/ others
- •96孔读板仪器。
- •自动洗板机。
- •移液器和枪头。建议使用多通道移液器/排枪。
- •Eppendorf 管。
- •额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
● 批内差/批间差/ others
| | Intra-Assay Precision | Inter-Assay Precision | ||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1278 | 6839 | 24157 | 1358 | 6350 | 25528 |
| Standard deviation | 62.62 | 465.05 | 1087.06 | 88.27 | 482.6 | 1225.34 |
| CV(%) | 4.90% | 6.80% | 4.50% | 6.50% | 7.60% | 4.80% |
