Thermo Scientific RevertAid链cDNA合成试剂盒包含了足够进行100次x20 µL反应的试剂。本品是以RNA为模板合成链cDNA的完整系统,可合成长达13kb的cDNA。本品含有RiboLock RNase抑制剂,能够有效保护RNA模板不受降解。
Thermo Scientific RevertAid链cDNA合成试剂盒配套提供oligo(dT)18引物和随机六聚体引物。oligo(dT)18引物可以选择性地与mRNA中的poly (A)尾结合。随机六聚体引物无需poly (A)尾,因此可转录mRNA 5’-端区域,也可以无poly (A)尾的RNA (如micoRNA)为模板合成cDNA。该试剂盒也可使用基因特异性引物。
应用链cDNA合成,作为RT-PCR和实时RT-qPCR的模板
构建全长cDNA文库
反义RNA合成
Full-length cDNA synthesis using the RevertAid First Strand cDNA Synthesis Kit
适用于(应用) | Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR) |
Optimal Reaction Temperature: | 42° C |
Product Line | RevertAid |
Reaction Format | Separate Components |
Reverse Transcriptase | RevertAid |
样品类型 | RNA |
足够用于 | 100 reactions |
- •The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 μM Forward GAPDH Primer, 10 μM Reverse GAPDH Primer, and nuclease-free water.
- •Store at -20°C.