人结肠癌细胞HCT116​​

人结肠癌细胞HCT116

人结肠癌细胞HCT116 是1979年BrattainM等分离建立的3株恶性细胞之一；产生CEA、角蛋白。在半固体琼脂糖培养基中形成克隆。HCT116在无胸腺的裸鼠有致瘤性，形成上皮样的肿瘤。

细胞名称：人结肠癌细胞

细胞简称：HCT116细胞

细胞别称：HCT-116; HCT.116; HCT_116; HCT116; CoCL2

产品货号：TCH-C185

种属来源：人

组织来源：结肠

特征：结肠癌

细胞形态：上皮细胞样

生长特性：贴壁生长

培养体系： McCoy's 5A+10%FBS+1%P/S

配套培养基货号：TCH-G185

传代比例：1:3-1:8，每2-3天换液一次

传代周期：48-72 h

培养条件：气相：95%空气+5%CO2，温度：37℃

冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存

质量检测：细菌、真菌、支原体检测均为阴性

实验耗材：细胞培养瓶，锥形摇瓶,细胞培养皿

实验室仪器：CO2培养箱

人结肠癌细胞HCT116 使用说明书

参考文献：

"Park SM, et al. The miR-200 family determines the epithelial phenotype of cancer cells by targeting the E-cadherin repressors ZEB1 and ZEB2. Genes Dev 22(7): 894-907, 2008. PubMed:

Schroy PC, et al. Detection of p21ras mutations in colorectal adenomas and carcinomas by enzyme-linked immunosorbent assay. Cancer 76: 201-209, 1995. PubMed: 8625092

Brattain MG, et al. Heterogeneity of malignant cells from a human colonic carcinoma. Cancer Res. 41: 1751-1756, 1981. PubMed: 7214343

Sun L, et al. Autocrine transforming growth factor-beta 1 and beta 2 expression is increased by cell crowding and quiescence in colon carcinoma cells. Exp. Cell Res. 214: 215-224, 1994. PubMed: 8082724

Santoro IM, Groden J. Alternative splicing of the APC gene and its association with terminal differentiation. Cancer Res. 57: 488-494, 1997. PubMed: 9012479

Brattain MG, et al. Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. J. Natl. Cancer Inst. 69: 767-771, 1982. PubMed: 6956756

Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064

Landers JE, et al. Translational enhancement of mdm2 oncogene expression in human tumor cells containing a stabilized wild-type p53 protein. Cancer Res. 57: 3562-3568, 1997. PubMed: 9270029

Kutchera W, et al. Protaglandin H synthase 2 is expressed abnormally in human colon cancer: evidence for a transcriptional effect. Proc. Natl. Acad. Sci. USA 93: 4816-4820, 1996. PubMed: 8643486

Wang R, et al. Cellular adherence elicits ligand-independent activation of the Met cell-surface receptor. Proc. Natl. Acad. Sci. USA 93: 8425-8430, 1996. PubMed: 8710887"