上海博研生物工程研究中心竭诚为您提供的产品和服务,期待您的来电,Elisa试剂盒,Elisa试剂盒说明书,Elisa试剂盒价格
产品名称:猪乙酰*(ACh)ELISA试剂盒优货销售
英文名称:PoFAine acetylcholine,ACH ElisaKit.
产品编号:BYS12616B
产品性状:液体
产品用途:科研实验
产品规格:96T/48T
ELISA实验原理: 用纯化的抗体包被微孔板,制成固相载体,往包被抗体的微孔中依次加入标本或者标准品、*化的抗体、HRP-标记的亲和素,经过*洗涤后用底物显色。底物在过氧化物酶的催化下转化成蓝色, 并在酸的作用下转化成zui终的黄色。 颜色的深浅和样品中的目标蛋白呈正相关。用酶标仪在(450nm)波长下测定测定O.D.值(吸光度),计算样品浓度。.
猪乙酰*(ACh)ELISA试剂盒优货销售 相关产品:
豚鼠免疫球蛋白E(IgE)试剂盒说明书,96T/48T*
豚鼠免疫球蛋白A(IgA)试剂盒说明书,96T/48T*
豚鼠基质金属蛋白酶抑制因子1(TIMP-1)试剂盒说明书,96T/48T*
豚鼠基质金属蛋白酶9(MMP-9)试剂盒说明书,96T/48T*
豚鼠肝细胞生长因子(HGF)试剂盒说明书,96T/48T*
豚鼠γ干扰素(IFN-γ)试剂盒说明书,96T/48T*
豚鼠端粒酶(TE)试剂盒说明书,96T/48T*
豚鼠促卵泡素(FSH)试剂盒说明书,96T/48T*
豚鼠肠脂肪酸结合蛋白(iFABP)试剂盒说明书,96T/48T*
豚鼠表皮生长因子(EGF)试剂盒说明书,96T/48T*
人PL7抗体/抗苏氨酰tRNA合成酶(PL7)试剂盒说明书,96T/48T*
人PL12抗体/抗丙氨酰tRNA合成酶(PL12/AlaRS)试剂盒说明书,96T/48T*
人抗Mi2抗体(anti-Mi2-Ab)试剂盒说明书,96T/48T*
人抗Ku抗体(anti-Ku-Ab)试剂盒说明书,96T/48T*
人抗神经元核抗体1型/抗Hu抗体(ANNA-1/Hu)试剂盒说明书,96T/48T*
人抗DNA酶B抗体(anti-DNase B)试剂盒说明书,96T/48T*
人抗BP180抗体(BP180-Ab)试剂盒说明书,96T/48T*
人抗BB抗体(BB-Ab)试剂盒说明书,96T/48T*
人幽门螺旋菌IgG(Hp-IgG)试剂盒说明书,96T/48T*
人可溶性血管内皮生长因子受体2(VEGFR-2/sFLK-1)试剂盒说明书,96T/48T*
The microtiter plate provided in ELISA Kit has been precoated with an antibody specific to the target protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated specific polyclonal antibody preparation,and Avidincon jugated to Horseradish Peroxidase(HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve