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NEXTFLEX®采用双索引条形码旨在制备多路单端和成对端基因组DNA文库,用于使用Illumina®平台进行测序。索引和流动细胞结合序列包含在NEXTFLEX®Unique Dual index条形码中,并在适配器连接过程中连接到样本插入物上。
Product Overview
The NEXTFLEX® Unique Dual Index Barcodes are designed to prepare multiplexed single and paired-end genomic DNA libraries for sequencing using Illumina® platforms. The index and flow cell binding sequences are contained within the NEXTFLEX® Unique Dual Index Barcodes and attached to the sample insert during adapter ligation. Sample pooling with NEXTFLEX® Unique Dual Index Barcodes allows the user to multiplex up to 384 samples when used in conjunction with other available sets of NEXTFLEX® Unique Dual Index Barcodes. Uniquely dual-indexed libraries are libraries prepared with adapters containing two eight base indexes: Index 1 (P7 Index) adjacent to the P7 strand, and Index 2 (P5 Index) adjacent to the P5 strand. None of the indexes found on any given NEXTFLEX® Unique Dual Index Barcode are used throughout the entire set, which prevents misassigned reads from appearing in final data sets. Each lot of the NEXTFLEX® Unique Dual Index Barcodes is functionally validated and tested for index purity by sequencing.
Kit Overview
This NEXTFLEX® Unique Dual Index Barcodes Kit contains 8, 24, or 96 uniquely dual- indexed barcoded DNA adapters in tubes or plate format for a total of 16, 48, or 192 reactions.
Kit Contents, Storage and Shelf Life
Note: The 16-reaction kit contains UDI barcodes 1-8, the 48-reaction kit contains UDI barcodes 1-24, and the 192 reaction kit contains UDI barcodes 1-96, 97-192, 193-288, or 289-384.
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