苏州阿尔法生物实验器材有限公司
苏州阿尔法生物实验器材有限公司
细胞名称：人T淋巴细胞白血病细胞（STR鉴定正确）细胞简称：Hut78细胞细胞别名：Hut 78; HUT 78; HuT-78; HUT-78; HuT78; Hut78;HUT78; NCI-H78货号：TCH-C218规格：1×10⁶cells/ T25 培养瓶Hut78细胞价格：1400元(干冰运输需另加200元干冰费)Hut78细胞培养基 货号：TCH-G218
Hut78细胞
细胞名称： 人T淋巴细胞白血病细胞（STR鉴定正确）
细胞简称：Hut78细胞
细胞别名： Hut 78; HUT 78; HuT-78; HUT-78; HuT78; Hut78;
HUT78; NCI-H78
货号：TCH-C218
规格：1×10⁶cells/ T25 培养瓶
价格：1400元 (干冰运输需另加200元干冰费)
种属来源：人
组织来源：皮肤
特征：塞扎里综合症
细胞形态：淋巴母细胞
生长特性：悬浮生长
培养体系：IMDM+20%FBS+1%P/S
细胞配套培养基 货号：TCH-G218
传代比例：1:3-1:6，每2-3天换液一次
传代周期：48-72 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
NCI-H78 细胞 可产生IL-2，TNF-α；表达IL-2受体
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Chen TR. Karyotypic derivation of H9 cell line expressing human immunodeficiency virus susceptibility. J. Natl. Cancer Inst. 84: 1922-1926, 1992. PubMed: 1460674
O'Connell MA, et al. Cellular proliferation and activation of NF kappa B are induced by autocrine production of tumor necrosis factor alpha in the human T lymphoma line HuT 78. J. Biol. Chem. 270: 7399-7404, 1995. PubMed: 7706285
Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991
Kolanus W, et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128
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Hut 78 was derived from peripheral blood of a patient with Sezary syndrome. The line has the properties of a mature human T cell with helper/inducer activity. It releases IL-2, and has receptors for IL-2. The growth rate is stimulated by IL-2. TNF alpha is an autocrine growth factor for Hut 78.
Cancer Cell 31:225-239(2017)
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