苏州阿尔法生物实验器材有限公司
苏州阿尔法生物实验器材有限公司
人结肠癌细胞HCT116是1979年BrattainM等分离建立的3株恶性细胞之一；产生CEA、角蛋白。在半固体琼脂糖培养基中形成克隆。HCT116在无胸腺的裸鼠有致瘤性，形成上皮样的肿瘤。
人结肠癌细胞HCT116
人结肠癌细胞HCT116 是1979年BrattainM等分离建立的3株恶性细胞之一；产生CEA、角蛋白。在半固体琼脂糖培养基中形成克隆。HCT116在无胸腺的裸鼠有致瘤性，形成上皮样的肿瘤。
细胞名称：人结肠癌细胞
细胞简称：HCT116细胞
细胞别称：HCT-116; HCT.116; HCT_116; HCT116; CoCL2
产品货号：TCH-C185
种属来源：人
组织来源：结肠
特征：结肠癌
细胞形态：上皮细胞样
生长特性：贴壁生长
培养体系： McCoy's 5A+10%FBS+1%P/S
配套培养基货号：TCH-G185
传代比例：1:3-1:8，每2-3天换液一次
传代周期：48-72 h
培养条件：气相：95%空气+5%CO2，温度：37℃
冻存条件：60%基础培养基+30%FBS+10%DMSO，液氮储存
质量检测：细菌、真菌、支原体检测均为阴性
实验耗材：细胞培养瓶，锥形摇瓶,细胞培养皿
实验室仪器：CO2培养箱
人结肠癌细胞HCT116 使用说明书
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