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Anti-TNXB抗体-腱糖蛋白X抗体简单介绍:
产品编号:BYK-18163R
英文名称:Anti-TNXB
中文名称:腱糖蛋白X抗体
别名:Ehlers Danlos like syndrome; Hexabrachion like protein; Hexabrachion-like protein; HXBL; NXB2; Tenascin X precursor; Tenascin XB; Tenascin XB1; Tenascin XB2; Tenascin-X; TENX; TENX_HUMAN; TN X; TN-X; TNX; TNXB; TNXB1; TNXB2; TNXBS; XB; XBS.
背景介绍:This gene encodes a member of the tenascin family of extracellular matrix glycoproteins. The tenascins have anti-adhesive effects, as opposed to fibronectin which is adhesive. This protein is thought to function in matrix maturation during wound healing, and its deficiency has been associated with the connective tissue disorder Ehlers-Danlos syndrome. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. It is one of four genes in this cluster which have been duplicated. The duplicated copy of this gene is incomplete and is a pseudogene which is transcribed but does not encode a protein. The structure of this gene is unusual in that it overlaps the CREBL1 and CYP21A2 genes at its 5' and 3' ends, respectively. Multiple transcript variants encoding different isoforms have been found for this gene.
产品规格:50ul;100ul;200ul;1ml,*
抗体来源 Rabbit or Mouse
克隆类型 Polyclonal or Monoclonal
交叉反应、产品应用 (具体交叉反应与产品应用请或添加客服直接索取)
性 状 Lyophilized or Liquid
浓 度 1mg/ml
Anti-TNXB抗体-腱糖蛋白X抗体产品信息:
石蜡切片免疫组化染色步骤
1、三步法(以SP试剂盒为例)
- 石蜡切片脱蜡至水
- 蒸馏水冲洗,PBS浸泡5分钟,如果采用抗原修复,可在此步后进行
- 3% H2O2 室温孵育5~10分钟,以消除内源性过氧化物酶的活性,PBS冲洗,2分钟×3次
- 5~10%正常山羊血清封闭,室温孵育10分钟。倾去血清,勿洗,滴加适当比例稀释的一抗或一抗工作液,37℃孵育1~2小时或4℃过夜
- PBS冲洗,2分钟×3次
- 滴加适当比例稀释的*标记二抗(1%BSA-PBS稀释),37℃孵育10~30分钟;或滴加第二代*标记二抗工作液,37℃或室温孵育10~20分钟
- PBS冲洗,2分钟×3次
- 滴加适当比例稀释的辣根酶标记链霉卵白素(PBS稀释),37℃孵育10~30分钟;或第二代辣根酶标记链霉卵白素工作液,37℃或室温孵育10~20分钟
- PBS冲洗,2分钟×3次
- 显色剂显色(DAB或AEC)
- 自来水充分冲洗,复染,脱水透明、封片
- 如果必要,可选用avdin封闭内源性*
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Anti-streptomycin *抗体
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