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淋巴细胞抗原6H抗体,Anti-LY6H抗体简单介绍:
产品编号:BYK-18455R
英文名称:Anti-LY6H
中文名称:淋巴细胞抗原6H抗体
别名:Ly-6H; LY 6H; Lymphocyte antigen 6 complex locus H; Lymphocyte antigen 6H; NMLY 6; NMLY6.
背景介绍:The Ly-6 (Lymphocyte antigen 6) alloantigens represent a family of phosphatidylinositol-anchored proteins that play a role in the process of T lymphocyte activation. Ly-6 family members share amino acid homology throughout a distinctive cysteine rich protein domain that incorporates O-linked carbohydrates. During hematopoiesis, murine Ly-6 molecules have unique patterns of tissue expression, from multipotential stem cells to lineage committed precursor cells, and on specific leukocyte subpopulations in the peripheral lymphoid tissues. Ly-6H (Lymphocyte antigen 6H), also known as NMLY6, is a 140 amino acid lipid-anchored membrane protein that belongs to the Ly-6 family. Highly expressed in brain with lower expression in pancreas, testis, colon and small intestine, Ly-6H participates in the activation of T lymphocytes. Overexpression of Ly-6H is detected in certain acute human leukemic cell lines (such as MOLT-3), suggesting a possible role for Ly-6H in carcinogenesis.
产品规格:50ul;100ul;200ul;1ml,*
抗体来源 Rabbit or Mouse
克隆类型 Polyclonal or Monoclonal
交叉反应、产品应用 (具体交叉反应与产品应用请或添加客服直接索取)
性 状 Lyophilized or Liquid
浓 度 1mg/ml
淋巴细胞抗原6H抗体,Anti-LY6H抗体产品信息:
合适的抗体稀释度
抗体的浓度是免疫染色的关键,如果抗体浓度过高,抗体分子过多于抗原决定簇,可导致抗体结合减少,产生阴性结果。此阴性结果并不一定是缺少抗原,而是由于抗体过量,这种现象类似于凝集反应中的前带效应(prozone effect)。因此,必须使用一系列稀释做“棋盘式效价滴定”,检测抗体的合适稀释度,以得到zui大强度的特异性染色和zui弱的背景染色。抗体稀释度应根据:1.抗体效价高,溶液中特异性抗体浓度越高,工作稀释度越高;2.一般讲,应用的抗体稀释度越大,温育时间越长;3.对于抗体与非特异性蛋白的结合,只有高稀释度时才能防止其非特异性背景染色;4.稀释用缓冲液的种类,标本的固定和处理过程等也可影响稀释度,所以合适的稀释度应根据具体情况测定。抗体的稀释主要是指*抗体,因为*抗体中特异性抗体合适的浓度是关键,应用高稀释度*抗体仅高亲和力的特异性染色反应,减少或消除其中交叉抗体反应。
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