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     六月，骄阳似火，暑气留恋。大街上，涌动的人流，五颜六色的衣妆，汇成横亘天地间的七色彩虹，描绘着生活美不胜收的诗情画意。

我们进入六月，一阵阵热浪扑面而来，整个世界都被烘烤得滚烫滚烫。也开始不再静下心来，烦躁，一触即发，但如果静心下来就会平淡如水，了无波痕。

而就是这样的六月我们的南京林业大学的老师使用上海沪鼎生物自主品牌ELISA试剂盒在《Overexpression of the LcCUC2-like》期刊上发表的SCI文献—— gene in Arabidopsis thaliana alters thecotyledon morphology and increasesrosette leaf number顺利通过，对生物科研方面有了新的的贡献。

精彩的文献不仅可以让我们更好的学习，还可以起到*作用，希望对正在做实验和写论文的科研小伙伴们有所帮助，通过文献我们可以更好的了解到产品，如果您也使用沪鼎品牌的ELISA试剂盒发表了SCI文献，欢迎您积极的参与我们的“fa论文赢奖学金"活动，小编等着你们的投稿哦，下一个高额领奖金的说不定就是你！

【文献标题】Overexpression of the LcCUC2-likegene in Arabidopsis thaliana alters thecotyledon morphology and increasesrosette leaf number

【DOI】Wen et al. (2022), PeerJ, DOI 10.7717/peerj.12615

【作者单位】南京林业大学 《Nanjing Forestry University》

【作者】Shaoying Wen1, Jiayu Li1

【产品文献支持】Auxin content measurement

Basal rosette leaves samples of 0.6 g were harvested from 3-week-old WT and LcCUC2L

transgenic A. thaliana plants and immediately frozen in liquid nitrogen. Determination

of indole-3-acetic acid (IAA) in A. thaliana leaves was performed by ELISA (Huding,

Shanghai, China). Three replicates were used for each sample.

影响因子(IF)= ：2.98

【出版期刊】：《PlantPhysiology and Biochemistry》

【产品原文引用】

Auxin content measurement

Basal rosette leaves samples of 0.6 g were harvested from 3-week-old WT and LcCUC2L

transgenic A. thaliana plants and immediately frozen in liquid nitrogen. Determination

of indole-3-acetic acid (IAA) in A. thaliana leaves was performed by ELISA (Huding,

Shanghai, China). Three replicates were used for each sample.

Histochemical staining to detect GUS activity

The β-Galactosidase Reporter Gene Staining Kit (Leagene) was used for GUS histochemical

staining in ProLcCUC2L transgenic and WT A. thaliana plants. In this study, seven-day-old

seedlings and fourteen-day-old seedlings were processed. Moreover, leaves at different

stages of development (10, 20 and 30 days of age) from plants planted in soil were

subjected to GUS staining. All materials were treated with GUS staining solution and

incubated overnight at 37 ◦C, followed by bleaching with 75% (v/v) ethanol.

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