Anti-ASIC1A酸敏感离子通道1抗体说明

Rabbit Anti-ASIC1/ASIC1A antibody 
Catalog Number : BYK-2586R        

Quantity size :   0.1ml  (1mg/ml   Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol) 
 
 Anti-ASIC1A酸敏感离子通道1抗体说明Background: Cation channel with high affinity for sodium, which is gated by extracellular protons and inhibited by the diuretic amiloride. Also permeable for Ca(2+), Li(+) and K(+). Generates a biphasic current with a fast inactivating and a slow sustained phase. Mediates glutamate-independent Ca(2+) entry into neurons upon acidosis. This Ca(2+) overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury. Heteromeric channel assembly seems to modulate channel properties. Functions as a postsynaptic proton receptor that influences intracellular Ca(2+) concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines. Modulates activity in the circuits underlying innate fear 
 
 Specificity :    

– Anti-ASIC1/ASIC1A is a Rabbit Polyclonal antibody unconjugated                  – specific for ASIC1/ASIC1A of Human, Mouse, Rat,  

– use for western blotting,elisa,immunoprecipitation and immunohistochemistry

– Protein G affinity chromatography purification, purity :>95%

– Isotype:  IgG

– mol wt:  58kDa 
 
Application :                                    

– WB=1:100-500

– ELISA=1:500-1000

– IHC-P=1:100-500  IHC-F=1:100-500

– ICC=1:100-500 IF=1:100-500

– Optimal working dilutions must be determined by the end user. 
 
 Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃ 
 
Important Note: This product as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.