Thorlabs Bergamo® II系列多光子显微镜
遵循显微镜设计 bi 须 符合样品要求的原则，创建了一套 wan 全 模块化的成像平台，可以满足多种实验需求。与传统的研究技术(例如使用微电极)相比，我们的多光子成像系统能够以更快速度、更大深度和更高强度同时读出和操纵神经元群。
Thorlabs Bergamo® II系列多光子显微镜
遵循显微镜设计 bi 须 符合样品要求的原则，创建了一套 wan 全 模块化的成像平台，可以满足多种实验需求。与传统的研究技术(例如使用微电极)相比，我们的多光子成像系统能够以更快速度、更大深度和更高强度同时读出和操纵神经元群。如下面的选项概览部分和右侧较下方的旋转Bergamo显微镜的五轴运动视频所示，我们开发了一个旋转主体，使显微镜可绕样品旋转。我们还提供各种刚性主体，其具有业界 ling,先 的7.74英寸高度，可围绕物镜提供较大的三维工作空间。
亮点
● 基于贝塞尔光束的快速体积成像
● 三光子成像
● 空间光调制器用于同时多点刺激
Thorlabs Bergamo® II系列多光子显微镜
基于贝塞尔光束的快速体积成像
Thorlabs与Howard Hughes yi,学院 以及Na Ji教授(加利福尼亚大学伯克利分校)合作，为我们的Bergamo®多光子激光扫描显微镜提供了贝塞尔光束模块。神经元活动的体内体积成像需要亚微米级空间分辨率和毫秒级时间分辨率。传统方法通过连续扫描衍射 ji 限 高斯光束以创建3D图像，而基于贝塞尔光束的多光子成像依靠轴向延伸的焦点来捕获体积图像。激发光束的扩展景深可创建3D体积的2D投影，从而 you 效 地将2D帧速率转换为3D体积速率。
单次贝塞尔扫描(左)可捕获与堆叠45个光学切片(右)而创建的高斯体积扫描相同的结构信息，从而将总扫描时间减少45倍。图像显示了300 μm x 300 μm范围内扫描的大脑切片。高斯堆叠的扫描深度由比例尺指示。样本由加州大学伯克利分校物理系Ji实验室的Qinrong Zhang博士和Matthew Jacobs提供。
规格
| Laser Scanning | |||
|---|---|---|---|
| Scan Path Wavelength Range | 450 - 1100 nm, 680 - 1300 nm, or 800 - 1800 nm | ||
| Scan Paths | Resonant-Galvo-Galvo Scanner, Galvo-Resonant Scanners, Galvo-Galvo Scanners, or Spatial Light Modulator; Single or Dual Scan Paths | ||
| Scan Speed | 8 kHz Resonant-Galvo-Galvo or Galvo-Resonant | 2 fps at 4096 x 4096 Pixels 30 fps at 512 x 512 Pixels 400 fps at 512 x 32 Pixels | |
| 12 kHz Resonant-Galvo-Galvo or Galvo-Resonant | 4.4 fps at 2048 x 2048 Pixels 45 fps at 512 x 512 Pixels 600 fps at 512 x 32 Pixels | ||
| Galvo-Galvo | 3 fps at 512 x 512 Pixels 48 fps at 512 x 32 Pixels 70 fps at 32 x 32 Pixels Pixel Dwell Time: 0.4 to 20 µs | ||
| Galvo-Galvo Scan Modes | Imaging: Line, Polyline, Square, or Rectangle Non-Imaging: Circle, Ellipse, Polygon, or Point | ||
| Field of View | FN40 Equivalent to 40 mm Diagonal Square (Max) at the Intermediate Image Plane >2.8 mm x 2.8 mm at the Sample plane with a 10X Objective FN20 Equivalent to 20 mm Diagonal Square (Max) at the Intermediate Image Plane >1.4 mm x 1.4 mm at the Sample plane with a 10X Objective | ||
| Scan Zoom | 1X to 16X (Continuously Variable) | ||
| Scan Resolution | Up to 2048 x 2048 Pixels (Bi-Directional) [Up to 1168 x 1168 Pixels for 12 kHz Scanners] Up to 4096 x 4096 Pixels (Unidirectional) [Up to 2336 x 2336 Pixels for 12 kHz Scanners] | ||
| Compatible Objective Threadings | M34 x 1.0, M32 x 0.75, M25 x 0.75, and RMS | ||
| Spatial Light Modulator (SLM) | ||||
|---|---|---|---|---|
| Stimulation Area (with a 16X Objective) | 600 µm x 600 µm (X and Y) ±150 µm (Z) | |||
| Resolution | 1024 pixels x 1024 pixels | |||
| Maximum Pattern Refresh Rate | 4 ms | |||
| Multiphoton Signal Detection | ||
|---|---|---|
| Epi Detection | Up to Four Ultrasensitive GaAsP PMTs, Cooled or Non-Cooled | |
| Forward-Direction Detection | Two Ultrasensitive GaAsP PMTs | |
| Maximum of Four PMTs Controlled by the Software at a Given Time | ||
| Collection Optics | 8°, 10°, or 14° Collection Angle (Angles Quoted When Using an Objective with a 20 mm Entrance Pupil) Easy-to-Exchange Emission Filters and Dichroic Mirrors | |
| Confocal Imaging | ||
|---|---|---|
| Motorized Pinhole Wheel with 16 Round Pinholes from Ø25 µm to Ø2 mm Two to Four Laser Lines (488 nm Standard; Other Options Range from 405 nm to 660 nm) Standard Multialkali or High-Sensitivity GaAsP PMTs Easy-to-Exchange Emission Filters and Dichroic Mirrors |
| Widefield Viewing | ||
|---|---|---|
| Manual or Motorized Switching Between Scanning and Widefield Modes Illumination Provided via LED or Liquid Light Guide C-Mount Threads for Scientific Cameras |
| Transmitted Light Imaging | ||
|---|---|---|
| Differential Interference Contrast (DIC) or Dodt Gradient Contrast Widefield or Laser Scanned Illumination Provided by Visible and/or NIR LEDs Compatible with Air or Oil Immersion Condensers |
| Three-Photon Imaging | ||
|---|---|---|
| Scan Optics for 800 - 1800 nm Range Achieve Reduced Background Scatter for Greater Sensitivity in Deep-Tissue Imaging |
| Volume Imaging Using Bessel Beams | ||
|---|---|---|
| 3D Volumetric Functional Imaging at Video Frame Rates Enhanced Temporal Resolution for Studying Internal Systems at Cellular Lateral Resolution In Vivo |
| Translation | ||||
|---|---|---|---|---|
| Microscope Body Rotation (Rotating Bodies Only) | 0° to 90° or -45° to +45° Around Objective Focus 0.1° Encoder Resolution | |||
| Coarse Elevator Base Z (Rotating Bodies Only) | 5" (127 mm) Total Travel; 1 µm Encoder Resolution | |||
| Fine Microscope Body X and Y | 2" (50.8 mm) Total Travel; 0.5 µm Encoder Resolution | |||
| Fine Microscope Arm Z | 1" (25.4 mm) Total Travel; 0.1 µm Encoder Resolution | |||
| Fine Z Focus | ||||
|---|---|---|---|---|
| Piezo Objective Scanner | Open Loop: 600 µm ± 10% Travel Range; 1 nm Resolution Closed Loop: 450 µm Travel Range; 3 nm Resolution | |||
| Vibrationless Remote Focus | 16 Discrete Steps ~400 µm Travel Range with a 10X Objective ~160 µm Travel Range with a 16X Objective | |||
森泉为您的科研事业添砖加瓦：
激光控制：激光电流源、激光器温控器、激光器控制、伺服设备与系统等等
探测器：光电探测器、单光子计数器、单光子探测器、CCD、光谱分析系统等等
定位与加工：纳米定位系统、微纳运动系统、多维位移台、旋转台、微型操作器等等
光源：半导体激光器、固体激光器、单频激光器、单纵模激光器、窄线宽激光器、光通讯波段激光器、CO2激光器、中红外激光器、染料激光器、飞秒超快激光器等等
光机械件：用于光路系统搭建的 gao 品 zhi 无应力光机械件，如光学调整架、镜架、支撑杆、固定底座等等
光学平台：主动隔振平台、气浮隔振台、实验桌、刚性工作台、面包板、隔振、隔磁、隔声综合解决方案等等
光学y件：各类晶体、光纤、偏转镜、反射镜、tou射镜、半透半反镜、滤光片、衰减片、玻片等等
染料：激光染料、荧光染料、光致变色染料、光致发光染料、吸收染料等等
