微生物冻干保护剂 返回列表页

该保护剂不含动物蛋白质！，微生物培养液与保护剂混合即可使用。使用该保护剂，细菌的存活率在90 ％以上，远远超过使用蔗糖和脱脂牛奶方法的细菌存活率。该试剂无菌过滤处理，包装是100毫升PET瓶。

使用方法：
1. Simple Culture Preservation:
Add an appropriate volume Lyophilized Buffer to a sterile vial(e.g., 100-500ul). Inoculate the buffer with 10ul of actively growing bacteria. All procedures must be performed aseptically. 
2.Preservation of Large Number of Cells:
Pellet cells from an actively growing culture by centrifugation. Decant the medium and resuspend the cell in an equal volume of Lyophilized Buffer. Transfer the cell solution to a sterile vial.

The freeze drying parameters must be determined for each freeze dryer. However the buffer are 
optimized to freeze drying on the following cycle.
Freezing:30 minutes from ambient to -40℃, hold for 1 hr.
Primary Drying: Increase temperature to -10℃, hold for 16 hours(large volumes 
may require longer reying times).
Secondary Drying:Increase temperature to 20℃, hold for 2 hr.