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血管壁细胞混合培养系统:
Dynamic Co-cultureSystem (Vascular Wall Cell Co-Culture) |
MK2000 |
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FEATURES |
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USAGES |
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Referencearticle |
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[ Effect on Endothelial Cell Gene Expression of ShearStress, Oxygen Concentration, and Low-Density Lipoprotein asStudied by a Novel Flow Cell Culture System ] |
FUNCTIONSSTRUCTURES |
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[SECTION OF CULTURE CELLCASETTE] | |
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SPECIFICAITONS |
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Structure | Size (mm) |
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Controller-Flow Controller, Display of DissolvedOxygen | 80 (W) x 340 (D) x 200 (H) |
Co-Culture Cell Unit (Diaphragm Pump, SolutionTank, Culture Cell Cassette) | 60 (W) x 250 (D) x 170 (H) |
Movies in Operation (Movie Zooming up, Movie No.2Autoclave) |
Wondows Media Player | Quick Time | ||
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Movie | Movie No.2 | Movie | Movie No.2 |
If you do not have Windows MediaPlayer, you can download from here--free of charge. | If you do not have Quick Time, you candownload from here--free of charge. |
[ Outsideview of Co-Culture Cell Unit ] | [ C*tion in the chamberof CO2 Incubator ] |
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[ Design Sample of CultureCell Cassette--customized design is possible.] |
(1) Branched CultureCell Cassette |
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(2) StraightCulture Cell Cassette |
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[ Hypoxia and Specifications] |
The air in the culture cell was replaced with Nitrogen gas so asnot to generate any gases inside while the culture solutioncontaining serum was circulated--for each 15ml of upper and lowerchambers. The measuring points of DO are shown in the left sketch. Hypoxiccondition (2-5%) appeared in some 10 minutes. |
(1) Tendency of descent velocity oflow concentration of Oxygen and minimum values through the pumpflow rate (cc/min) | |
Pump flow rate: 3-30 cc/min The maximum descent velocity of lowconcentration of Oxygen is 15cc/min. |
(2) Effectiveness of descendingspeed of low concentration of Oxygen and the minimum Oxygen valuesthrough the pump flow rate and the filter pore size | |
Pump flow rate: 3, 9 cc/min
In case that the large flow rate is set, there is atendency that the larger pore size of filter, the lesser effectiveexchange rate of DO. |
(3) Dissolved Oxygen values each atupper inlet and outlet, lower inlet and outlet | |
Pump flow rate: 3 cc/min
The performance of an oxygen concentration gradientin about 30 min is confirmed. |
ANCILLARYACCESSORIES |
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The following accessories are optional at extra charge.
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PRICES |
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Main Body | ||
Model | Price | |
Dynamic Coculture System (Vascular Wall CellCo-Culture) MK2000 | The customized types are available. | |
Consumables | ||
Available cell in market | Quantity per package (ea) | Price (¥) |
Cell culture insert | 48 | ¥25,200 |
NOTE: The specifications and theappearance subject to change without previous notification. Thecustomized systems also are possible according to the customerdemands, on a case by case basis . |
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