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血管壁细胞混合培养系统

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具体成交价以合同协议为准

产品型号MK2000

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血管壁细胞混合培养系统

血管壁细胞混合培养系统:

Dynamic Co-cultureSystem (Vascular Wall Cell Co-Culture)
MK2000
CO-CULTURE FLOWSYSTEM ALLOWS CIRCULATION EVEN SMALL CULTUREMEDIA.
"In Vitro" flow system allows culture,under similar conditions to live organs, with a filtering culturecup. The culture cup is partitioned with the filter into upper andlower parts where about 10cc culture solution can circulateindividually for a long time. This new culture system enablesresearcher to analyze the initial stage of arteriosclerosislesions, to induce the gene expression of other vascular diseases,and to examine the permeability of new drugs into vascularendothelial cells, because this system simulates possible physicalstimulus being given to the live organs such as the flow to thevascular endothelial cells, the hypoxia to the smooth muscles,etc.



*FEATURES
  • While co-culturing both vascular endothelial and smooth musclecells in a filtering culture cup, the environmental stimulus can beprovided with them.
  • Environmental stimulus similar to live organs is given tovarious kinds of cells, and then respective gene, and proteinlinked with them are analyzed at molecular scale routinely.
  • Saves the culture media and shortens the time of collection andrefinery of target samples, as only a little quantity of culturesolution is circulated.
  • Filtering culture cups are available on the market, andpossible to co-culture previously, which allows you to obtain thegood repeatability by handling and seeding cellseasily.



*USAGES
  1. Analysis of gene expression (Human vascular endothelial cells)in all respects caused by the combined stimulus of flow, Oxygenconcentration gradient and Low-density lipoprotein (LDL)

  2. Analysis of anti-inflammation's symptoms caused by flow stress(Human vascular endothelial cells, vascular endothelial cells oftranscription factors knockout mouse)




*Referencearticle

[ Effect on Endothelial Cell Gene Expression of ShearStress, Oxygen Concentration, and Low-Density Lipoprotein asStudied by a Novel Flow Cell Culture System ]
Free Radic Biol Med. 2004 Sep 1;37(5):682-94.




*FUNCTIONSSTRUCTURES
  • The culture cell of this system is divided into upper and lowercells by the partition of a permeable PET membrane.
  • A cell culture insert for 6-well plates manufactured by BD isused as the filtering culture cup.
  • The cells of membrane can be exposed with the physical power offlow generated by the circulation of 10cc of culture solution.
  • As Nitrogen gas can be replaced with the air of the culturecell, an oxygen concentration gradient is performed by thecirculation of culture solution having a very low dissolved oxygenconcentration--its partial pressure of oxygen is about 2 to 5 %.
  • An observation window is provided for an inverted or a co-focusmicroscope.
  • A diaphragm pump with the minimum differential pressuremaintains the culture solutions of upper and lower cupwell-balanced for a long time--the operation is available overnightwithout an operator present.
  • Possible of monitoring the partial pressure of dissolvedoxygen.
  • The drug injection or the sampling after the drug injection isavailable via a drug sampling port.
  • All contaminated parts can be autoclaved.


[SECTION OF CULTURE CELLCASETTE]
SECTION OF CULTURE CELL CASETTE
  • Type of cells in a culture cell depends on R&D.
  • A filtering culture cup, cell preparation and seeding cell--arenot included in the system.
  • When the low oxygen concentration is desired in operation, anoptional incubator and the gases exchange system arenecessary.



*SPECIFICAITONS

Structure

Size (mm)

Controller-Flow Controller, Display of DissolvedOxygen

80 (W) x 340 (D) x 200 (H)

Co-Culture Cell Unit (Diaphragm Pump, SolutionTank, Culture Cell Cassette)

60 (W) x 250 (D) x 170 (H)



Movies in Operation (Movie Zooming up, Movie No.2Autoclave)
Wondows Media Player Quick Time
Movie Movie No.2 Movie Movie No.2
If you do not have Windows MediaPlayer, you can download from here--free of charge.
Windows Media Player
If you do not have Quick Time, you candownload from here--free of charge.
Quick Time


[ Outsideview of Co-Culture Cell Unit ] [ C*tion in the chamberof CO2 Incubator ]
??????? CO2????????????????
  1. Culture cell cassette
  2. Solution tank
  3. Diaphragm Pump


[ Design Sample of CultureCell Cassette--customized design is possible.]
(1) Branched CultureCell Cassette
Branched Culture Cell Cassette
  • Low shear stress (about 0.2 to 2 dynes/cm2)
  • Seeding cells in the filtering culture cup
  • Long keeping the oxygen low concentration gradient
(2) StraightCulture Cell Cassette
Straight Culture Cell Cassette
  • High shear stress (about 5 to 15 dynes/cm2)
  • Seeding cells at outside of filter is to be loaded high shearstress


[ Hypoxia and Specifications]

The air in the culture cell was replaced with Nitrogen gas so asnot to generate any gases inside while the culture solutioncontaining serum was circulated--for each 15ml of upper and lowerchambers.

The measuring points of DO are shown in the left sketch. Hypoxiccondition (2-5%) appeared in some 10 minutes.

Hypoxia and Specifications

(1) Tendency of descent velocity oflow concentration of Oxygen and minimum values through the pumpflow rate (cc/min)
Tendency of descent velocity of low concentration of Oxygen and minimum values through the pump flow rate (cc/min)

Pump flow rate: 3-30 cc/min
Filter pore size: 0.4 micro meter dia.
N2 flow rate:50 cc/min
Measuring point: (1)

The maximum descent velocity of lowconcentration of Oxygen is 15cc/min.



(2) Effectiveness of descendingspeed of low concentration of Oxygen and the minimum Oxygen valuesthrough the pump flow rate and the filter pore size
Effectiveness of descending speed of low concentration of Oxygen and the minimum Oxygen values through the pump flow rate and the filter pore size

Pump flow rate: 3, 9 cc/min
Filter pore size: 0.4, 3.0 micro meter dia.
N2 flow rate:50 cc/min
Measuring point: (1)

In case that the large flow rate is set, there is atendency that the larger pore size of filter, the lesser effectiveexchange rate of DO.



(3) Dissolved Oxygen values each atupper inlet and outlet, lower inlet and outlet
Dissolved Oxygen values each at upper inlet and outlet, lower inlet and outlet

Pump flow rate: 3 cc/min
Filter pore size: 0.4, micro meter dia.
N2 flow rate:50 cc/min
Measuring point: (1) to (4)

The performance of an oxygen concentration gradientin about 30 min is confirmed.




*ANCILLARYACCESSORIES

The following accessories are optional at extra charge.

  1. CO2 incubator
  2. Gas cylinders
  3. When the low oxygen concentration is desired in operation, anoptional incubator and the gas exchange system are necessary,although they are not shown in the photos.
  4. The system does NOT include vascular wall cells. Please contactus regarding the method of culture.



*PRICES

Main Body

Model

Price

Dynamic Coculture System (Vascular Wall CellCo-Culture) MK2000

The customized types are available.
It depends upon the requirements of the customers. Specificinquires welcome .

Consumables

Available cell in market

Quantity per package (ea)

Price (¥)

Cell culture insert
(6-well type 0.45µm)
Manufactured by BD (Nippon Becton Dickinson)

48

¥25,200
(Cell culture insert only: ¥24,000)


NOTE: The specifications and theappearance subject to change without previous notification. Thecustomized systems also are possible according to the customerdemands, on a case by case basis .

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