Anti-PCGF4/BMI1-组蛋白相关Bmi1抗体说明书

来源：上海博研生物工程研究中心 2016年04月05日 11:03

Anti-PCGF4/BMI1-组蛋白相关Bmi1抗体说明书

Rabbit Anti-PCGF4/BMI1 antibody

Catalog Number : BYK-2999R
Quantity size : 0.1ml (1mg/ml,Lyophilized or Liquid.Buffer=0.01M PBS,ph 7.4 with 10 mg/ml BSA and 0.1% Sodium azide.Reconstitute with 0.1ml sterile distilled water.)

background: The Bmi1 oncogene induces omerase activity and immortalizes human mammary epithelial cells. Bmi1 extends the replicative life span of human fibroblasts by suppressing the p16-dependent senescence pathway.

Polycomb group (PcG) genes are involved in the maintenance of cellular memory through epigenetic chromatin modifications. Recent studies have implicated a role for PcG genes in the self-renewal of hematopoietic stem cells (HSCs), a process in which cellular memory is maintained through cell division. Among the PcG genes, Bmi1 plays a central role in the inheritance of stemness, and its forced expression promotes HSC self-renewal. These findings highlight the importance of epigenetic regulation in HSC self-renewal and identify PcG genes as potential targets for therapeutic HSC manipulation. Involved in maintaining the transcriptionally repressive state of genes. Modifies chromatin, rendering it heritably changed in its expressibility.

Specificity :

Anti-PCGF4/BMI1 is a rabbit Polyclonal antibody unconjugated
specific for PCGF4/BMI1 of Human, Mouse, Rat
use for western blotting,elisa,immunoprecipitation and immunohistochemistry
Protein G affinity chromatography purification, purity :>95%
Isotype: IgG
mol wt: 37kDa

Application :

WB=1:100-500
Immunohistocry 1:100-500
ELISA= 1:500-1000
IHC-P=1:100-500 IHC-F=1:100-500
IF=1:100-500

Optimal working dilutions must be determined by the end user.

Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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